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Agriculture and Food

Sustainable Chemical Production through Microbial Cell Factories

In silico analysis of five industrial microorganisms identifies optimal strains and metabolic engineering strategies for producing 235 valuable chemicals.

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The global need for sustainable chemical production has become increasingly important due to climate change and the depletion of fossil fuels. In response to this environmental challenge, microbial cell factories are gaining attention as eco-friendly platforms for producing chemicals using renewable resources. To maximize production efficiency, metabolic engineering technologies have become crucial tools. However, difficulties in selecting suitable microbial strains and optimizing complex metabolic pathways continue to pose significant obstacles to practical industrial applications.

Researchers from KAIST’s Department of Chemical and Biomolecular Engineering have made a groundbreaking discovery by comprehensively evaluating the production capabilities of various industrial microbial cell factories using in silico simulations. This approach has enabled the identification of the most suitable microbial strains for producing specific chemicals, as well as optimal metabolic engineering strategies.

In the past, researchers attempted to determine the best strains and efficient metabolic engineering strategies through extensive biological experiments and meticulous verification processes. However, this approach required substantial time and costs. The introduction of genome-scale metabolic models (GEMs) has enabled systematic analysis of metabolic fluxes via computer simulations, revolutionizing both strain selection and metabolic pathway design.

Professor Lee’s team evaluated the production capabilities of five representative industrial microorganisms – E. coli, S. cerevisiae, B. subtilis, C. glutamicum, and P. putida – for 235 bio-based chemicals. Using GEMs, they calculated both the maximum theoretical yields and the maximum achievable yields under industrial conditions for each chemical. The team specifically proposed strategies such as introducing heterologous enzyme reactions derived from other organisms and exchanging cofactors used by microbes to expand metabolic pathways.

These strategies were shown to increase yields beyond the innate metabolic capacities of the microorganisms, resulting in higher production of industrially important chemicals such as mevalonic acid, propanol, fatty acids, and isoprenoids. Moreover, the team suggested strategies for improving microbial strains to maximize the production of various chemicals by analyzing metabolic fluxes in silico.

The researchers quantitatively identified the relationships between specific enzyme reactions and target chemical production, as well as the relationships between enzymes and metabolites, determining which enzyme reactions should be up- or down-regulated. Through this, the team presented strategies not only to achieve high theoretical yields but also to maximize actual production capacities.

Dr. Gi Bae Kim, the first author of this paper from the KAIST BioProcess Engineering Research Center, explained that by introducing metabolic pathways derived from other organisms and exchanging cofactors, it is possible to design new microbial cell factories that surpass existing limitations. The strategies presented in this study will play a pivotal role in making microbial-based production processes more economical and efficient.

In conclusion, this research serves as a key resource in the field of systems metabolic engineering, reducing difficulties in strain selection and pathway design, and enabling more efficient development of microbial cell factories. This technology is expected to greatly contribute to the future development of technologies for producing various eco-friendly chemicals, such as biofuels, bioplastics, and functional food materials.

Agriculture and Food

Feat of ‘Dung-Gineering’: Turning Cow Manure into a Sustainable Resource

A new technique to extract tiny cellulose strands from cow dung and turn them into manufacturing-grade cellulose, currently used to make everything from surgical masks to food packaging, has been developed.

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A groundbreaking technique has been developed by researchers from UCL and Edinburgh Napier University to extract tiny cellulose strands from cow manure and turn them into manufacturing-grade cellulose. This innovation, published in The Journal of Cleaner Production, has the potential to create cellulose materials more cheaply and cleanly than some current manufacturing methods.

The advance is a prime example of circular economy, which aims to minimize waste and pollution by reusing and repurposing resources wherever possible. Cellulose is one of the world’s most commonly used manufacturing materials, found naturally in plant cell walls. It was first used to create synthetic materials in the mid-19th century, including photographic film.

Today, cellulose can be found in everything from cling film to surgical masks, paper products, textiles, foods, and pharmaceuticals. Although it can be extracted organically, it is often produced synthetically using toxic chemicals. The new technique, called horizontal nozzle-pressurized spinning, is an energy-efficient process that doesn’t require high voltages like other fiber production techniques.

The researchers say implementing this technology would be a win-win situation for manufacturers, dairy farmers, and the environment. Dairy farm waste, such as cow manure, is a threat to the environment and humans, especially through waterway pollution, greenhouse gas emissions when it decomposes, and the spread of pathogens. By putting this problematic waste product to good use, the technology could be a huge boost to the global dairy farming industry.

The research team is currently seeking opportunities to work with dairy farmers to take advantage of the technology and scale it up. With existing pressurized spinning machines adaptable to the new process, adapting to the logistics of sourcing and transporting cow dung might be the greater challenge.

However, the environmental and commercial benefits would be significant. As animal waste becomes a growing problem globally, this innovation offers a beacon of hope for sustainable resource management. The team is excited about the potential impact on ecosystems and human health, making it a groundbreaking achievement in “dung-gineering.”

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Agriculture and Food

The Dark Side of Cannabis Use in Pregnancy

A systematic evidence review finds that consuming cannabis while pregnant appears to increase the odds of preterm birth, low birth weight and infant death.

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The Dark Side of Cannabis Use in Pregnancy

A recent systematic review has raised significant concerns about the risks associated with consuming cannabis while pregnant. The study, conducted by researchers at Oregon Health & Science University, analyzed 51 observational studies involving over 21 million people to examine the potential adverse effects of cannabis use during pregnancy.

The findings are alarming: consuming cannabis appears to increase the odds of preterm birth, low birth weight, and infant death. These results are consistent with definitive evidence in nonhuman primate models exposed to THC, the main psychoactive compound in cannabis.

The placenta, a vital organ responsible for supplying oxygen and nutrients to the fetus, is particularly affected by cannabis use during pregnancy. Research has shown that cannabis can lead to decreased blood flow and reduced availability of oxygen in the placenta, ultimately affecting fetal development and growth.

While many pregnant women may view cannabis as a harmless substance due to its increasingly widespread legalization, the reality is far more concerning. Cannabis remains a Schedule 1 substance under federal law, and its use during pregnancy is not recommended by medical professionals.

The lead author of the study, Dr. Jamie Lo, emphasizes the importance of prioritizing fetal health over any perceived benefits of cannabis use during pregnancy. “Abstinence is ideal,” she notes, “but it’s not realistic for many patients.” In such cases, a harm-reduction approach can be recommended, advising pregnant women to reduce their cannabis consumption and frequency to minimize potential risks.

The research, part of the Systematically Testing the Evidence on Marijuana (STEM) project, highlights the need for more comprehensive studies on the effects of cannabis use during pregnancy. By understanding these risks, healthcare providers can better counsel expectant mothers and promote healthier outcomes for both mother and child.

Sources:

* The study was published in JAMA Pediatrics.
* Funding for this research was provided by the Office of Rural Health in the Veterans Health Administration of the U.S. Department of Veterans Affairs and the National Institute on Drug Abuse of the National Institutes of Health.

Key Takeaways:

* Consuming cannabis during pregnancy appears to increase the odds of preterm birth, low birth weight, and infant death.
* The placenta is particularly affected by cannabis use during pregnancy, leading to decreased blood flow and reduced availability of oxygen.
* A harm-reduction approach can be recommended for pregnant women who cannot abstain from cannabis use.
* More comprehensive studies are needed to fully understand the effects of cannabis use during pregnancy.

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Agriculture and Food

Ancient Andes Society Used Hallucinogens to Strengthen Social Order

Snuff tubes uncovered at Chavin de Huantar in Peru reveal how leaders used mystical experiences to cement their power.

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In the highlands of Peru, two thousand years before the Inca empire rose to power, a lesser-known society known as the Chavín Phenomenon flourished. This enigmatic culture left behind a rich legacy of art, architecture, and materials that spanned modern-day Peru. Through innovative agriculture, craft production, and trade, Chavín built a growing social order and laid the foundations for hierarchical societies among the Andes’ highest peaks.

But one of their most powerful tools wasn’t farming – it was access to altered states of consciousness. A recent study has uncovered the earliest-known direct evidence of psychoactive plant use in the Peruvian Andes, shedding light on the ritualistic practices that shaped this ancient society.

Researchers from the University of Florida, Stanford University, and South American institutions discovered ancient snuff tubes carved from hollow bones at the heart of monumental stone structures at Chavín de Huántar, a prehistoric ceremonial site. By conducting chemical and microscopic analyses, they revealed traces of nicotine from wild relatives of tobacco and vilca bean residue, a hallucinogen related to DMT.

These substances were not used for personal visions but as part of tightly controlled rituals that reinforced the social hierarchy. Unlike communal hallucinogenic use common in other ancient cultures, Chavín’s rituals were exclusive, with snuff tubes found in private chambers within massive stone structures that held only a handful of participants at a time.

“Taking psychoactives was not just about seeing visions,” said Daniel Contreras, Ph.D., an anthropological archaeologist at UF and co-author of the study. “It was part of a tightly controlled ritual, likely reserved for a select few, reinforcing the social hierarchy.”

These experiences were likely profound and even terrifying, with the supernatural world feeling like a force beyond comprehension. By controlling access to these altered states, Chavín’s rulers established a potent ideology that convinced their people that their leadership was intertwined with mystical power and part of the natural order.

The study also uncovered trumpets made from conch shells and chambers seemingly designed to enhance awe-inducing musical performances. These rituals extended beyond the use of psychedelics, creating immersive experiences that reinforced belief systems and social structures.

“One of the ways that inequality was justified or naturalized was through ideology – through the creation of impressive ceremonial experiences that made people believe this whole project was a good idea,” Contreras said.

Their study, published in the Proceedings of the National Academy of Sciences, helps solve a century-old mystery about this site. Since its first excavation over a hundred years ago, Chavín has been seen as related to both earlier, more egalitarian societies and the mountain-spanning empires ruled by powerful elites that came later.

Controlled access to mystical experiences explains this major social transition, a finding only made possible by decades of intense excavations and advanced analytical methods. As Contreras noted, “It’s exciting that ongoing excavations can be combined with cutting-edge archaeological science techniques to get us closer to understanding what it was like to live at this site.”

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