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Extreme Survival

Unlocking the Secrets of Bat Immunity: A New Platform for Studying Viral Defense Mechanisms

Bats are known as natural hosts for highly pathogenic viruses such as MERS- and SARS-related coronaviruses, as well as the Marburg and Nipah viruses. In contrast to the severe and often fatal disease outcomes these viruses cause in humans, bats generally do not show obvious signs of viral illness following infection. An international research team has developed an innovative organoid research platform that allowed them to closely investigate the cellular antiviral defense mechanisms of mucosal epithelial tissues of bats. The results could pave the way for the development of new therapies against viral diseases.

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Researchers have long been fascinated by bats’ ability to host highly pathogenic viruses without showing obvious signs of illness. Now, a team led by Dr. Max Kellner and Prof. Josef Penninger has developed an innovative organoid research platform that allows them to closely investigate the cellular antiviral defense mechanisms of mucosal epithelial tissues in bats. The results have been published in Nature Immunology and could pave the way for the development of new therapies against viral diseases.

The researchers used tissue samples from Egyptian fruit bats, which are natural hosts of the highly pathogenic Marburg virus, to generate organoids that mimic the initial viral exposure – mucosal surfaces serve as entry points for many viruses into the body. The team successfully infected both bat and human airway organoids with the Marburg virus in a high-security Biosafety Level 4 (S4) laboratory.

Compared to human models, bat organoids exhibited a significantly higher baseline antiviral immune activity even before infection. This likely enables bats to control viral replication early in infected mucosal tissues, while human cells are less effective at recognizing the Marburg virus in the early stages of infection, allowing uncontrolled replication and spread throughout the body.

The researchers discovered that type III interferons play a crucial role in the mucosal antiviral immunity of Egyptian fruit bats. After infection with various zoonotic viruses, bat organoids exhibited an exceptionally strong production of these interferons. The team confirmed the strong antiviral activity of these interferons through additional stimulation experiments and genetic modifications.

The results suggest that bats can effectively prevent uncontrolled viral replication through a combination of innate immune processes, thereby avoiding viral diseases. Understanding the resilience mechanisms of these animals against highly pathogenic viruses and the evolutionary adaptation of their immune systems is essential for developing antiviral therapies and fighting future pandemics.

In addition to the novel insights into the antiviral mechanisms of bat mucosal tissues, bat organoids will offer an innovative platform for more precise studies of the complex biology of bats at the genetic and molecular levels. The research team plans to further develop the organoid models in terms of complexity and make them available to the scientific community.

Only by working together can we understand the complex mechanisms that evolution has shaped in animals like bats, and from this, develop new approaches for combating and treating viral diseases.

Acid Rain

The Hidden Impact of Anoxic Pockets on Sandy Shores

Some microbes living on sand grains use up all the oxygen around them. Their neighbors, left without oxygen, make the best of it: They use nitrate in the surrounding water for denitrification — a process hardly possible when oxygen is present. This denitrification in sandy sediments in well-oxygenated waters can substantially contribute to nitrogen loss in the oceans.

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The Hidden Impact of Anoxic Pockets on Sandy Shores

Human activities have dramatically increased nitrogen inputs into coastal seas, leading to a significant amount of this human-derived nitrogen being removed by microorganisms in coastal sands through denitrification. However, research has shown that this process can also occur in oxygenated sands, and scientists from the Max Planck Institute for Marine Microbiology in Bremen, Germany, have now revealed how this happens.

The scientists used a method called microfluidic imaging to visualize the diverse and uneven distribution of microbes and the oxygen dynamics on extremely small scales. “Tens of thousands of microorganisms live on a single grain of sand,” explains Farooq Moin Jalaluddin from the Max Planck Institute for Marine Microbiology. The researchers could show that some microbes consume more oxygen than is resupplied by the surrounding pore water, creating anoxic pockets on the surface of the sand grains.

These anoxic microenvironments have so far been invisible to conventional techniques but have a dramatic effect: “Our estimates based on model simulations show that anaerobic denitrification in these anoxic pockets can account for up to one-third of the total denitrification in oxygenated sands,” says Jalaluddin.

The researchers calculated how relevant this newly researched form of nitrogen removal is on a global scale and found that it could account for up to one-third of total nitrogen loss in silicate shelf sands. Consequently, this denitrification is a substantial sink for anthropogenic nitrogen entering the oceans.

In conclusion, the hidden impact of anoxic pockets on sandy shores has been revealed by scientists, highlighting the importance of these microenvironments in removing nitrogen from coastal seas and emphasizing the need to consider them when assessing the overall nitrogen budget of our planet.

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Cholesterol

“Breaking Down Barriers to IBS Relief: The Mediterranean Diet’s Promising Pilot Study Results”

In a comparative pilot study, the Mediterranean diet and the low FODMAP diet both provided relief for patients with IBS.

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Breaking Down Barriers to IBS Relief: The Mediterranean Diet’s Promising Pilot Study Results

A groundbreaking pilot study from Michigan Medicine researchers has revealed that the Mediterranean diet may provide symptom relief for individuals with irritable bowel syndrome (IBS). Conducted on patients diagnosed with either IBS-D (diarrhea) or IBS-M (mixed symptoms of constipation or diarrhea), this innovative study aimed to compare the efficacy of two popular dietary interventions: the Mediterranean diet and the low FODMAP diet.

The research team randomly assigned participants into two groups, one following the Mediterranean diet and the other adhering to the restriction phase of a low FODMAP diet. The primary endpoint was an FDA-standard 30% reduction in abdominal pain intensity after four weeks. Notably, while both diets showed symptom relief, the low FODMAP group experienced greater improvement measured by both abdominal pain intensity and IBS symptom severity score.

The study’s findings are significant, given that a majority of patients with IBS prefer dietary interventions over medication. Furthermore, restrictive diets like low FODMAP can be difficult to adopt due to their complexity and potential for nutrient deficiencies. In contrast, the Mediterranean diet is already well-established as a beneficial eating pattern for overall health.

The study’s lead author, Prashant Singh, MBBS, emphasized that “restrictive diets can be costly and time-consuming” and may even lead to disordered eating behaviors. The researchers believe that further studies comparing the long-term efficacy of the Mediterranean diet with the low FODMAP reintroduction phase are necessary to fully understand its potential as an effective intervention for patients with IBS.

The University of Michigan’s William Chey, M.D., senior author on the paper, added that “this study adds to a growing body of evidence which suggests that a Mediterranean diet might be a useful addition to the menu of evidence-based dietary interventions for patients with IBS.”

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Biochemistry Research

Breaking Barriers with Frozen Ethanol: Researchers Revolutionize Biological Lithography

Imagine drawing on something as delicate as a living cell — without damaging it. Researchers have made this groundbreaking discovery using an unexpected combination of tools: frozen ethanol, electron beams and purple-tinted microbes. By advancing a method called ice lithography, the team was able to etch incredibly small, detailed patterns directly onto fragile biological surfaces.

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Imagine being able to draw intricate patterns on living cells without damaging them – a feat previously thought impossible. Researchers at the University of Missouri have achieved this groundbreaking discovery using an innovative method called ice lithography, which relies on frozen ethanol, electron beams, and purple-tinted microbes.

The traditional liquid-based lithography process can harm delicate materials like carbon nanotubes and biological membranes. Mizzou’s ice-based approach uses a layer of frozen ethanol to protect the material’s surface while creating patterns, making it possible to work with fragile biological materials that would normally be damaged substantially.

Led by Professor Gavin King, the team used Halobacterium salinarum, a tiny microorganism that makes a purple protein capable of capturing sunlight and turning it into energy. This microbe’s ability to efficiently convert light into energy makes it a promising candidate for developing new kinds of power sources.

To test their new ethanol-ice-based method, researchers used a scanning electron microscope to create tiny patterns in the frozen layer. The parts of the ice that weren’t hit by the beam were sublimed away, while the pattern – now a solid material – was left behind.

The team’s findings bring together the fields of biology, chemistry, physics, and space science, and could transform how scientists work with the tiniest building blocks of life – molecules, proteins, and atoms. The discovery has major implications for future research, including the possibility of using these delicate purple membranes to create solar panels.

The interdisciplinary teamwork behind this breakthrough is a testament to the power of collaboration in scientific discovery. Each lab contributed a different piece of the puzzle, making it possible to achieve something previously thought impossible.

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