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Artificial Intelligence

Accelerating Evolution: The Power of T7-ORACLE in Protein Engineering

Researchers at Scripps have created T7-ORACLE, a powerful new tool that speeds up evolution, allowing scientists to design and improve proteins thousands of times faster than nature. Using engineered bacteria and a modified viral replication system, this method can create new protein versions in days instead of months. In tests, it quickly produced enzymes that could survive extreme doses of antibiotics, showing how it could help develop better medicines, cancer treatments, and other breakthroughs far more quickly than ever before.

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The accelerated evolution engine known as T7-ORACLE has revolutionized the field of medicine and biotechnology by allowing researchers to evolve proteins with new or improved functions at an unprecedented rate. This breakthrough was achieved by Scripps Research scientists who have developed a synthetic biology platform that enables continuous evolution inside cells without damaging the cell’s genome.

Directed evolution is a laboratory process where mutations are introduced, and variants with improved function are selected over multiple cycles. Traditional methods require labor-intensive steps and can take weeks or more to complete. In contrast, T7-ORACLE accelerates this process by enabling simultaneous mutation and selection with each round of cell division, making it possible to evolve proteins continuously and precisely inside cells.

T7-ORACLE circumvents the bottlenecks associated with traditional approaches by engineering E. coli bacteria to host a second, artificial DNA replication system derived from bacteriophage T7. This allows for continuous hypermutation and accelerated evolution of biomacromolecules, making it possible to evolve proteins in days instead of months.

To demonstrate the power of T7-ORACLE, researchers inserted a common antibiotic resistance gene into the system and exposed E. coli cells to escalating doses of various antibiotics. In less than a week, the system evolved versions of the enzyme that could resist antibiotic levels up to 5,000 times higher than the original.

The broader potential of T7-ORACLE lies in its adaptability as a platform for protein engineering. Scientists can insert genes from humans, viruses, or other sources into plasmids and introduce them into E. coli cells, which are then mutated by T7-ORACLE to generate variant proteins that can be screened or selected for improved function.

This could help scientists more rapidly evolve antibodies to target specific cancers, evolve more effective therapeutic enzymes, and design proteases that target proteins involved in cancer and neurodegenerative disease. The system’s ease of implementation, combined with its scalability, makes it a valuable tool for advancing synthetic biology.

The research team is currently focused on evolving human-derived enzymes for therapeutic use and tailoring proteases to recognize specific cancer-related protein sequences. In the future, they aim to explore the possibility of evolving polymerases that can replicate entirely unnatural nucleic acids, opening up possibilities in synthetic genomics.

Artificial Intelligence

The Real-Life Kryptonite Found in Serbia – A Game-Changer for Earth’s Energy Transition

Deep in Serbia’s Jadar Valley, scientists discovered a mineral with an uncanny resemblance to Superman’s Kryptonite both in composition and name. Dubbed jadarite, this dull white crystal lacks the glowing green menace of its comic book counterpart but packs a punch in the real world. Rich in lithium and boron, jadarite could help supercharge the global transition to green energy.

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The discovery of jadarite, a rare and fascinating mineral, has been hailed as “Earth’s kryptonite twin” due to its similarities to the fictional substance from the comic books. Found in the Jadar Valley of Serbia by exploration geologists from Rio Tinto in 2004, this sodium lithium boron silicate hydroxide mineral has immense potential for Earth’s energy transition away from fossil fuels.

Initially, jadarite didn’t match any known mineral at the time and was identified after analysis by the Natural History Museum in London and the National Research Council of Canada. It was officially recognized as a new mineral in 2006. While it shares some similarities with kryptonite, including its chemical formula LiNaSiB₃O₇(OH), jadarite is a much less supernatural dull white mineral that fluoresces pinkish-orange under UV light.

According to Michael Page, a scientist with Australia’s Nuclear Science and Technology Organisation (ANSTO), “the real jadarite has great potential as an important source of lithium and boron.” In fact, the Jadar deposit where it was first discovered is considered one of the largest lithium deposits in the world, making it a potential game-changer for the global green energy transition.

The work that ANSTO does focuses on how critical minerals like jadarite can be utilized to support Australian industry in a commercial capacity. They have produced battery-grade lithium chemicals from various mineral deposits, including spodumene, lepidolite, and even jadarite, ensuring that Australian miners receive the support they need to meet the challenges of the energy transition.

As the world continues to transition towards renewable energy sources, jadarite’s potential as a key component in this process cannot be overstated. Its discovery is a testament to human ingenuity and our ability to find innovative solutions to complex problems.

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Artificial Intelligence

Revolutionizing Quantum Computing with an Ultra-Thin Chip

Researchers at Harvard have created a groundbreaking metasurface that can replace bulky and complex optical components used in quantum computing with a single, ultra-thin, nanostructured layer. This innovation could make quantum networks far more scalable, stable, and compact. By harnessing the power of graph theory, the team simplified the design of these quantum metasurfaces, enabling them to generate entangled photons and perform sophisticated quantum operations — all on a chip thinner than a human hair. It’s a radical leap forward for room-temperature quantum technology and photonics.

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In the quest for practical quantum computers and networks, photons have emerged as promising carriers of information at room temperature. However, controlling and coherently manipulating these particles within optical devices has proven notoriously difficult due to their inherently noisy nature. To overcome this hurdle, researchers from Harvard’s John A. Paulson School of Engineering and Applied Sciences have developed an innovative solution – a metasurface-based quantum photonics processor.

This groundbreaking device is the result of Federico Capasso’s research team, led by graduate student Kerolos M.A. Yousef. By harnessing the power of specially designed metasurfaces, flat devices etched with nanoscale light-manipulating patterns, they have created an ultra-thin upgrade for quantum-optical chips and setups.

One of the primary advantages of this design is its ability to miniaturize an entire optical setup into a single metasurface. This results in a robust and scalable system that offers numerous benefits, including cost-effectiveness, simplicity of fabrication, and low optical loss. The work has significant implications for quantum sensing, enabling “lab-on-a-chip” capabilities for fundamental science.

To tackle the complex mathematical challenges associated with this design, the researchers drew upon graph theory – a branch of mathematics that uses points and lines to represent connections and relationships. This allowed them to visually determine how photons interfere with each other and predict their effects in experiments.

The resulting paper was a collaboration with Marko Loncar’s lab, which provided expertise and equipment necessary for the project. Neal Sinclair, a research scientist on the team, expressed excitement about this approach, stating that it could efficiently scale optical quantum computers and networks – their biggest challenge compared to other platforms like superconductors or atoms.

This groundbreaking research received support from federal sources, including the Air Force Office of Scientific Research (AFOSR), under award No. FA9550-21-1-0312. The work was performed at the Harvard University Center for Nanoscale Systems.

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Artificial Intelligence

Google’s Deepfake Hunter: Exposing Manipulated Videos with a Universal Detector

AI-generated videos are becoming dangerously convincing and UC Riverside researchers have teamed up with Google to fight back. Their new system, UNITE, can detect deepfakes even when faces aren’t visible, going beyond traditional methods by scanning backgrounds, motion, and subtle cues. As fake content becomes easier to generate and harder to detect, this universal tool might become essential for newsrooms and social media platforms trying to safeguard the truth.

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In an era where manipulated videos can spread disinformation, bully people, and incite harm, researchers at the University of California, Riverside (UCR), have created a powerful new system to expose these fakes. Amit Roy-Chowdhury, a professor of electrical and computer engineering, and doctoral candidate Rohit Kundu, teamed up with Google scientists to develop an artificial intelligence model that detects video tampering – even when manipulations go far beyond face swaps and altered speech.

Their new system, called the Universal Network for Identifying Tampered and synthEtic videos (UNITE), detects forgeries by examining not just faces but full video frames, including backgrounds and motion patterns. This analysis makes it one of the first tools capable of identifying synthetic or doctored videos that do not rely on facial content.

“Deepfakes have evolved,” Kundu said. “They’re not just about face swaps anymore. People are now creating entirely fake videos – from faces to backgrounds – using powerful generative models. Our system is built to catch all of that.”

UNITE’s development comes as text-to-video and image-to-video generation have become widely available online. These AI platforms enable virtually anyone to fabricate highly convincing videos, posing serious risks to individuals, institutions, and democracy itself.

“It’s scary how accessible these tools have become,” Kundu said. “Anyone with moderate skills can bypass safety filters and generate realistic videos of public figures saying things they never said.”

Kundu explained that earlier deepfake detectors focused almost entirely on face cues. If there’s no face in the frame, many detectors simply don’t work. But disinformation can come in many forms. Altering a scene’s background can distort the truth just as easily.

To address this, UNITE uses a transformer-based deep learning model to analyze video clips. It detects subtle spatial and temporal inconsistencies – cues often missed by previous systems. The model draws on a foundational AI framework known as SigLIP, which extracts features not bound to a specific person or object. A novel training method, dubbed “attention-diversity loss,” prompts the system to monitor multiple visual regions in each frame, preventing it from focusing solely on faces.

The result is a universal detector capable of flagging a range of forgeries – from simple facial swaps to complex, fully synthetic videos generated without any real footage. It’s one model that handles all these scenarios,” Kundu said. “That’s what makes it universal.”

The researchers presented their findings at the high-ranking 2025 Conference on Computer Vision and Pattern Recognition (CVPR) in Nashville, Tenn. Their paper, led by Kundu, outlines UNITE’s architecture and training methodology.

While still in development, UNITE could soon play a vital role in defending against video disinformation. Potential users include social media platforms, fact-checkers, and newsrooms working to prevent manipulated videos from going viral.

“People deserve to know whether what they’re seeing is real,” Kundu said. “And as AI gets better at faking reality, we have to get better at revealing the truth.”

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