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Biology

Yeast Revolutionizes Human Protein Production with DNase1 Breakthrough

The protein DNase1 is one of the oldest biological agents in history: It has been on the market since 1958 and is now used, among other things, to treat cystic fibrosis. However, it takes considerable effort to produce it in immortalized hamster cells. This process is also costly. It would be far more cost-effective to produce it with undemanding yeast cells.

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The production of human proteins has long been a costly and labor-intensive process, often relying on mammalian cells. However, a team led by Dr. Markus Napirei at Ruhr University Bochum has successfully produced the human protein DNase1 using yeast cells, a breakthrough that could revolutionize the field.

DNase1 is an enzyme used to treat cystic fibrosis and other conditions, but its production in mammalian cells has been limited by high costs and effort. The new method uses Pichia pastoris, a type of yeast fungus, to produce the protein, which can be stably integrated into the yeast genome and released as desired.

“This is the result of years of work, and could lay the groundwork for the manufacture of human DNase1 in yeast as a biological agent,” says Dr. Napirei. The research was published in PLOS ONE on April 29, 2025.

The advantages of using yeast cells over mammalian cells are clear: cost-effective culture conditions, high reproduction rates without the need to immortalize cells, and lower susceptibility to pathogens. In his doctoral thesis, Jan-Ole Krischek successfully expressed human DNase1 in Pichia pastoris, cleaned it, and characterized it for the first time.

One of the surprising findings was that the yeast produced considerably less human DNase1 than the mouse DNase1 used as a guide, despite sharing 82 percent of their primary structure. This is partly due to specific folding behaviors of the two proteins, explains Dr. Napirei.

DNase1 has been used for over 60 years to treat various conditions, including cystic fibrosis. The enzyme degrades cell-free DNA that can induce symptoms of illness. Inhaled DNase1 liquifies DNA-laden bronchial mucus, making it easier to cough up. Its potential use in other pathological processes is vast, particularly in the removal of neutrophil extracellular traps (NETs) and microthrombi that contain high levels of NET components.

Dr. Napirei suggests that DNase1 could be used to better dissolve microthrombi containing DNA, an application currently being explored in clinical studies. Another potential use is in dissolving thrombosis of a cerebral artery in the case of ischemic strokes.

This breakthrough has significant implications for the production and use of human proteins, particularly DNase1. The ability to produce this enzyme using yeast cells could lead to more cost-effective and efficient treatment options for patients, ultimately improving their quality of life.

Agriculture and Food

The Ozone Secret: Extending Mango Storage Life by 28 Days

Mango lovers and growers alike may soon rejoice: scientists at Edith Cowan University have found that a simple dip in ozonated water can drastically extend the shelf life of mangoes by up to two weeks while reducing spoilage. This technique, called aqueous ozonation, helps prevent chilling injuries that typically occur during cold storage, a long-standing challenge in mango preservation.

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The article highlights groundbreaking research conducted at Edith Cowan University, where scientists have discovered an innovative way to extend the storage life of mangoes by up to 28 days. Led by Dr Mekhala Vithana, the study reveals that dipping mangoes in ozonated water for 10 minutes before cold storage significantly reduces chilling injury and extends shelf life.

Mango lovers rejoice! The research is a game-changer for growers and traders alike, as it reduces food loss during storage and provides a longer market window. With the global demand for fruits and vegetables on the rise, this eco-friendly technology could minimize post-harvest losses of mangoes and reduce waste in Australia.

Traditionally, mangoes are stored at 13 degrees Celsius for up to 14 days, but this temperature is not cold enough to prevent chilling injury. Prolonged storage below 12.5 degrees causes physiological disorders that damage the fruit skin and lead to decreased marketability and significant food waste.

The study tested aqueous ozonation technology on Australia’s most widely produced mango variety, Kensington Pride, and found that dipping the mango in ozonated water for 10 minutes prior to cold storage at 5 degrees Celsius extended shelf life up to 28 days with much less chilling injury. This breakthrough could revolutionize the way we store mangoes and reduce food waste.

Dr Vithana emphasizes that aqueous ozonation is a cost-effective, controlled-on-site technology that can be used in commercial settings. The researchers hope to conduct further studies on other varieties of mangoes to test their responsiveness and achieve further reduction in chilling injury for extended cold storage.

As we continue to explore innovative solutions to reduce food waste, the ozone secret could hold the key to extending mango storage life by 28 days, benefiting both growers and consumers alike.

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Agriculture and Food

The Tiny Condos of Fiji’s Ant Plant: A Key to Harmonious Coexistence Among Unrelated Symbionts

High in Fiji s rainforest, the ant plant Squamellaria grows swollen tubers packed with sealed, single-door apartments. Rival ant species nest in these chambers, fertilizing their host with nutrient-rich waste while never meeting face-to-face. When researchers sliced open the walls, fatal battles erupted, confirming that the plant s compartmentalized architecture prevents war and sustains the partnership. CT scans of the tubers unveiled a meticulously isolated maze, showcasing evolution s clever fix for keeping multiple, unrelated houseguests peacefully productive.

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The Fiji ant plant, Squamellaria, has long been studied for its remarkable ability to form symbiotic relationships with ants. But what makes this relationship truly unique is the way the plant provides separate “condos” for each ant species, preventing conflicts that could arise from competition for resources. Researchers from Washington University in St. Louis and Durham University in the United Kingdom have made a groundbreaking discovery about the secrets behind this harmonious coexistence.

The study, published in Science, reveals that compartmentalization is the key to mitigating conflicts between unrelated symbionts. By creating separate chambers within its tubers, Squamellaria prevents ant colonies from coming into contact with each other, thereby reducing competition for resources and eliminating deadly conflicts.

“We were able to visualize directly what theory has long predicted – that unrelated partners would conflict by competing for host resources,” said Susanne S. Renner, senior author of the study. “But here we also have a simple, highly effective evolutionary strategy to mitigate these conflicts: compartmentalization.”

The researchers used computed-tomography scanning and 3D modeling to visualize the tubers’ internal structure and understand how the plant enables multiple ant species to live together in harmony. They found that removing the partition walls between the chambers resulted in immediate conflict and high worker mortality, emphasizing the importance of compartmentalization.

This discovery has significant implications for our understanding of symbiotic relationships and the ecology and evolution of species interactions. It highlights the remarkable ability of Squamellaria to adapt to its environment and form mutually beneficial relationships with ants, even when faced with conflicting interests.

The study’s findings also shed light on a long-standing problem in ecological theory – how unrelated partners can form long-term mutualistic relationships despite competing for host resources. By providing separate compartments, Squamellaria has evolved an effective solution to this problem, allowing multiple ant species to coexist peacefully and benefiting from each other’s presence.

In conclusion, the tiny condos of Fiji’s ant plant have unlocked a secret to harmonious coexistence among unrelated symbionts, offering new insights into the complex relationships between species.

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Biochemistry Research

The Whispering Womb: Uncovering the Secret Language of Embryonic Cells

Scientists found that embryonic skin cells “whisper” through faint mechanical tugs, using the same force-sensing proteins that make our ears ultrasensitive. By syncing these micro-movements, the cells choreograph the embryo’s shape, a dance captured with AI-powered imaging and computer models. Blocking the cells’ ability to feel the whispers stalls development, hinting that life’s first instructions are mechanical. The discovery suggests hearing hijacked an ancient force-sensing toolkit originally meant for building bodies.

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The human body begins as a single cell that multiplies and differentiates into thousands of specialized cells. Researchers at the Göttingen Campus Institute for Dynamics of Biological Networks (CIDBN) and the Max Planck Institute have made a groundbreaking discovery: embryonic cells “listen” to each other through molecular mechanisms previously known only from hearing.

Using an interdisciplinary approach combining developmental genetics, brain research, hearing research, and theoretical physics, the researchers found that in thin layers of skin, cells register the movements of their neighboring cells and synchronize their own tiny movements with those of the others. This coordination allows groups of neighboring cells to pull together with greater force, making them highly sensitive and able to respond quickly and flexibly.

The researchers created computer models of tissue development, which showed that this “whispering” among neighboring cells leads to an intricate choreography of the entire tissue, protecting it from external forces. These findings were confirmed by video recordings of embryonic development and further experiments.

Dr. Matthias Häring, group leader at the CIDBN, explained that using AI methods and computer-assisted analysis allowed them to examine about a hundred times more cell pairs than was previously possible in this field, giving their results high accuracy.

The mechanisms revealed in embryonic development are also known to play a role in hearing, where hair cells convert sound waves into nerve signals. The ear is sensitive because of special proteins that convert mechanical forces into electrical currents. This discovery suggests that such sensors of force may have evolved from our single-celled ancestors, which emerged long before the origin of animal life.

Professor Fred Wolf, Director of the CIDBN, noted that future work should determine whether the original function of these cellular “nanomachines” was to perceive forces inside the body rather than perceiving the outside world. This phenomenon could provide insights into how force perception at a cellular level has evolved.

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