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Revolutionizing Drug Development with Minimal Mice: A Breakthrough in Antibody Testing

New active ingredients such as antibodies are usually tested individually in laboratory animals. Researchers have now developed a technology that can be used to test around 25 antibodies simultaneously in a single mouse. This should not only speed up the research and development pipeline for new drugs, but also hugely reduce the number of laboratory animals required.

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The development of new drugs is a complex process that requires extensive testing on animals before they can be approved for human use. Researchers at the University of Zurich have made a groundbreaking discovery that could significantly reduce the number of laboratory animals required in this process.

The team, led by Markus Seeger from the Institute of Medical Microbiology and Johannes vom Berg from the Institute of Laboratory Animal Science, has developed a technology that allows them to test up to 25 antibodies simultaneously in a single mouse. This innovation is set to speed up the research and development pipeline for new drugs while also reducing the number of laboratory animals required.

Antibodies are proteins that specifically bind to certain structures on cells or molecules. They are used in many modern medicines, including treatments for cancer, autoimmune diseases, and infectious diseases. However, testing antibodies individually in animal models is a time-consuming process that requires a large number of laboratory animals.

The new technology uses protein fragments called flycodes to mark each antibody individually. These flycodes act as barcodes, allowing researchers to separate and analyze the individual antibodies from a complex mixture. The results show that this method delivers high-quality preclinical data on the investigated antibodies, reducing the need for multiple animal tests.

One of the most significant advantages of this technology is its potential to reduce the number of laboratory animals required in drug development. According to the researchers, this new method could reduce the number of animals required by a factor of up to 100. This not only speeds up the research and development process but also contributes to reducing animal suffering.

The researchers demonstrated that their flycode technology does not compromise the efficacy of the antibodies in living organisms. They tested the antibodies against cancer cells, and the targeted accumulation worked correctly even when combined with other antibodies. The team also used flycodes to analyze the properties and data for a series of 80 drug-like synthetic biomolecules, known as sybodies.

The breakthrough has significant implications for the pharmaceutical industry, where preclinical tests account for a large proportion of animals used. By reducing the number of animal tests required, this technology can advance preclinical discovery pipelines more efficiently in the future.

Animals

A “Roadmap” to Understanding the Fruit Fly Brain: Breakthrough Study Reveals Comprehensive Insights into Entire Nervous System

Researchers have gained comprehensive insights into the entire nervous system of the fruit fly (Drosophila melanogaster). The study describes in detail the neurons that span the entire nervous system of the adult fruit fly. The researchers also compared the complete set of neural connections (the connectome) in a female and a male specimen — and identified differences.

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The fruit fly (Drosophila melanogaster) has long been a model organism for scientists studying genetics, development, and behavior. However, despite its importance, the intricacies of the fruit fly’s nervous system have remained somewhat of a mystery – until now. Researchers at Leipzig University and other institutions have made a groundbreaking discovery, publishing a study in Nature that provides comprehensive insights into the entire nervous system of the adult fruit fly.

For the first time, scientists have mapped out the neural connections (the connectome) in a female and a male specimen, revealing differences between the two sexes. This breakthrough is a significant step forward in understanding the complex interactions within the fruit fly’s brain and nervous system.

The study, led by Dr. Katharina Eichler from Leipzig University, involved analyzing three connectomes: one female brain data set and two nerve cord data sets (one male, one female). The researchers used light microscopy to identify all neurons in the neck of the fruit fly that could be visualized using this technique.

This allowed them to analyze the circuits formed by these cells in their entirety. When comparing male and female neurons, the scientists identified sex-specific differences for the first time. They found previously unknown cells that exist only in one sex and are absent in the other.

One notable example is a descending neuron known as aSP22, which communicates with neurons present only in females. This finding provides an explanation for the behavioral differences observed when this neuron is active: female flies extend their abdomen to lay eggs, while males curl theirs forward to mate.

The study’s findings are significant not only because they provide a comprehensive overview of the fruit fly connectome but also because they offer a “roadmap” for future research. By understanding the intricate connections within the nervous system, scientists can design more intelligent experiments to investigate the function of individual neurons or entire circuits – saving time and resources.

As Eichler notes, now that the technical challenges in analyzing the fruit fly’s nervous system have been overcome, her research group is working on two new data sets covering the entire central nervous system of both a female and a male specimen. This continued research will undoubtedly shed more light on the complexities of the fruit fly brain and its implications for our understanding of nervous systems in general.

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Animals

The Social Parrot: Unpacking the Connection Between Communication and Group Living

For social animals, communication is a key that unlocks the benefits of group living. It’s well known that animals with more complex social lives tend to have more intricate ways of communicating, from the clicks and whistles of dolphins to the calls of primates. While this pattern is found broadly in many species, a new study on wild parrots drills deep into the social and vocal lives of individual birds. Researchers analyzing the social networks of monk parakeets in Spain have uncovered how an individual’s social ties shapes the calls these birds make.

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The urban parks of Barcelona, Spain, are home to a thriving colony of tropical monk parakeets. These vibrant green birds, native to South America, have adapted well to their new European environment. As they live in large groups, they communicate with each other using an array of distinct sounds – offering scientists a unique window into understanding the intricate relationships between individual social connections and vocal variety.

For animals that live in complex societies, communication is the key that unlocks the benefits of group living. From dolphins’ clicks and whistles to primates’ calls, it’s well-known that species with more intricate social lives tend to have more diverse ways of communicating. However, a recent study on wild parrots has drilled deeper into the social and vocal lives of individual birds.

Researchers at the Max Planck Institute of Animal Behavior spent two years closely observing 337 monk parakeets in Spain, documenting their social lives and recording over 5,599 vocalizations – an astonishing number that provides a wealth of data for analysis. By examining these calls in terms of repertoire diversity (the variety of sounds a bird can make) and contact-call diversity (how unique this specific type of call is), the team was able to uncover some fascinating insights.

The study revealed that individual parakeets living in larger groups did indeed produce more variable repertoires of sounds. Interestingly, female parakeets had a more diverse repertoire than males – an unusual finding for birds. This suggests that females may be the more social sex, and their vocalizations reflect this.

Social network analysis showed that parakeets with more central positions in the social structure – those that were potentially more influential in the group – tended to have more diverse vocal repertoires. In other words, the most social individuals seemed to have a better vocabulary than less social individuals.

The researchers also found that close friends who allowed each other to approach within pecking distance sounded less like each other, as if they were trying to sound unique in their little gang. These findings offer clues about the evolution of complex communication, including human language.

As Simeon Smeele, the first author of the study, notes, “The next big step is to better understand what each of the sounds mean – a real mammoth task, since most of the social squawking happens in large groups with many individuals talking at the same time!” The study provides a crucial foundation for further research into the intricate relationships between communication and group living in animals.

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Ancient DNA

New Drone-Assisted 3D Mapping Revolutionizes Dinosaur Fossil Dating in Alberta’s Dinosaur Provincial Park

A new study is reshaping how scientists date dinosaur fossils in Alberta’s Dinosaur Provincial Park (DPP). Using advanced drone-assisted 3D mapping, researchers have uncovered significant variations in a key geological marker, challenging long-standing methods of determining the ages of dinosaur fossils.

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The ancient landscapes of Alberta’s Dinosaur Provincial Park have long been a treasure trove for paleontologists seeking to unravel the mysteries of the past. However, a new study from McGill University is about to change the game when it comes to dating dinosaur fossils in this UNESCO World Heritage Site.

For decades, scientists have relied on a key geological marker – the contact between the Oldman and Dinosaur Park Formations – as a reference point to estimate the ages of fossil quarries. This method involves comparing how high or low a fossil site is relative to that boundary. But, according to researchers Alexandre Demers-Potvin and Professor Hans Larsson, this approach has significant limitations.

Their study, published in Palaeontologia Electronica, reveals that the Oldman-Dinosaur Park Formation boundary fluctuates in elevation by as much as 12 meters over short distances. This means that estimates of individual fossil ages could be off by a considerable margin – potentially altering our understanding of when different species lived.

To address these uncertainties, Demers-Potvin and Larsson employed advanced drone-assisted 3D mapping techniques to capture high-resolution images of a key fossil site in the park. By processing these images through structure-from-motion photogrammetry, the team created a precise 3D model of the terrain which is geolocated with GPS coordinates measured in the field.

The results are promising: this new dating method might be more dependable than relying on elevation measurements, and could lead to more accurate reconstructions of ancient ecosystems. By mapping sedimentary layers over a broader area, researchers may develop a much clearer picture of biodiversity shifts in an ancient terrestrial ecosystem.

“We’ve essentially shown that the dating method used for decades in Dinosaur Provincial Park may not be as reliable as previously thought,” said Demers-Potvin. “This opens the door to a more refined approach for understanding how different dinosaur species succeeded one another over time.”

The implications of this study are far-reaching, and could have significant impacts on our understanding of Earth’s history and past biodiversity changes. By refining our methods for dating dinosaur fossils, we can gain a deeper appreciation for the complex ecosystems that existed in the ancient world – and may even inform present and future life on our planet.

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